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Novel Calcium Indicators & Dyes for Outstdanding Fluorescence

See all standard Calcium Dyes and Calcium Assay Kits

 

QUEST Fluo-8TM

The Brightest & Fastest Green Calcium Indicator



Fluo-3 and Fluo-4 are most commonly used among the visible light-excitable calcium indicators. However, Fluo-3 AM and Fluo-4 AM are only moderately fluorescent in live cells upon esterase hydrolysis, and require harsh cell loading conditions to maximize their cellular calcium responses.

Fluo-8® dyes are developed to improve cell loading and calcium response, while maintaining the convenient Fluo-3 and Fluo-4 spectral wavelength:

 

Brighter
  • 4x Brighter than Fluo-3 AM
  • 2x Brighter than Fluo-4 AM
Faster
  • Dye Loading at Room Temperature
  • Loading Time 30-45 Minutes Only
Robust
  • Enables Calcium Assays with Difficult Receptors and Cell Lines
Convenient
  • Excitation at 490 nm
  • Fluorescence at 520 nm
  • FITC Filter Set-Compatible


We offer a set of outstanding Fluo-8® reagents with different calcium binding affinities:

Fluo-8: Kd = 389 nM
Fluo-8H: Kd = 232 nM
Fluo-8L: Kd = 1.86 µM
Fluo-8FF: Kd = 10 µM

For price quotations and any product information please contact us at +972 (0) 89417118, or email us at info@advansys.co.il.

For detailed protocols please click here.

 

 

QUEST Rhod-4TM


The Brightest Red Calcium Indicator

 

  • More Sensitive than Rhod-2
     
  • 10x Brighter than Rhod-2 in CHO & HEK cells
     
  • Faster that Rhod-2
     
  • Localized in the Cytoplasm


Rhod-2 is most commonly used among the red fluorescent calcium indicators. However, Rhod-2 AM is only moderately fluorescent in live cells upon esterase hydrolysis, and has very small cellular calcium responses.

Quest Rhod-4™ has been developed to improve Rhod-2 cell loading and calcium response while maintaining the spectral wavelength of Rhod-2. In CHO and HEK cells Quest Rhod-4™ AM has cellular calcium response that is 10 times more sensitive than Rhod-2 AM.

For price quotations and any product information please contact us at +972 (0) 89417118, or email us at info@advansys.co.il.

For detailed protocols please click here.


ADVANSYS is the exclusive distributor of AAT Bioquest (USA) in Israel, experts in Fluorescence, Luminescence, Absorption technologies.